13194s 1 ab 2750869 western blot 6 primary anti superoxide dismutase 1 antibody rabbit  (Cell Signaling Technology Inc)

Journal: Theranostics

Article Title: The combination of calreticulin-targeting L-ASNase and anti-PD-L1 antibody modulates the tumor immune microenvironment to synergistically enhance the antitumor efficacy of radiotherapy

doi: 10.7150/thno.90376

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: Recombinant anti-SOD2/MnSOD Ab , Abcam/ab68155 , 1:1000 dilution.

Techniques: Recombinant

Journal: International Journal of Molecular Sciences

Article Title: Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells

doi: 10.3390/ijms24043561

Figure Lengend Snippet: Effects of Fu on the antioxidant response in Cis-treated UB/OC-2 cells. ( a ) The free radical-scavenging activity of Fu (0.25–2 mg/mL) was determined via DPPH assays. Cells were cultured with Fu (2 mg/mL) for 2 h, followed by stimulation with Cis (5 μg/mL) for 24 h. ( b ) Protein expression levels of Nrf2 in the nuclear and cytosolic fractions were measured via Western blot analysis. Lamin B1 and β-actin were used as internal controls for nuclear and cytosolic fractions, respectively. ( c ) The mRNA expression levels of Nrf2 , Ho1 , and Nqo1 were determined using reverse transcription-quantitative polymerase chain reaction. ( d ) Determination of Nrf2, HO-1, and NQO1 protein expression levels was based on Western blotting analysis. ( e ) Expression of SOD1, SOD2, catalase, and GPx protein expression levels were determined using Western blot analysis. β-actin was used as a loading control. The results are expressed as the mean ± standard deviation. n = 3 per group. * p < 0.05 vs. the control group; # p <0.05 vs. the Cis group. Fu, fucoidan; Cis, cisplatin; Nrf2, NF-E2–related factor 2; HO-1, heme oxygenase 1; NQO1, NAD(P)H Quinone Dehydrogenase 1; SOD1, superoxide dismutase 1; SOD2, superoxide dismutase 2; GPx, glutathione peroxidase.

Article Snippet: The membrane was blocked in 5% skim milk in Tris-buffered saline for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies: Bcl-2 (1:1000; Cell Signaling Technology, Beverly, MA, USA), Bax (1:1000; Cell Signaling Technology), Caspase-9 (1:1000; Cell Signaling Technology), Caspase-3 (1:1000; Cell Signaling Technology), PARP (1:1000; Cell Signaling Technology), Nrf2 (1:1000, Abcam), HO-1 (1:1000; Proteintech, Manchester, UK), NQO1 (1:1000; Abcam), SOD1 (1:10,000; Abcam), SOD2 (1;1000; Elabscience Biotechnology Inc., Houston, TX, USA), catalase (1:1000; Elabscience Biotechnology Inc.), and GPx (1:1000; Elabscience Biotechnology Inc.).

Techniques: Activity Assay, Cell Culture, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Standard Deviation